Mouse Secondary Antibodies
Rockland Immunochemicals Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP - 18-8817-30
Mouse TrueBlot ULTRA has been reported for use in IP and WB. Mouse IgG TrueBlot ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution. Specific conditions for each protein and antibody combination should be specifically optimized by the end user. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
Jackson Immuno Research Labs Alkaline Phosphatase AffiniPure® Mouse Anti-Rabbit IgG (H+L) 0.5ml
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody exhibits inherent minimal cross-reaction to mouse serum proteins and has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, goat, mouse and sheep serum proteins. The antibody may cross-react with immunoglobulins from other species.Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
ACROBiosystems Mouse LRRC15 / LIB Protein Fc Tag MALS verified
Mouse LRRC15 / LIB Protein Fc Tag MALS verified
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Total α-Synuclein Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total α-Synuclein Detection Kit, 100 Assay Points
Santa Cruz Biotechnology MOUSE ANTI-RAB IGG HRP CONJ
mouse anti-rabbit IgG HRP, Conventional Secondary Antibodies for Western Blotting, supplied at 200microgram in 0.5 ml volume, to be used in Western blotting at a dilution of 1:500 - 1:10000
Jackson Immuno Research Labs MOUSE ANTI RAT IGG
AffiniPure Mouse Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs,Ms,Gt,Rb Sr Prot)
Jackson Immuno Research Labs Peroxidase AffiniPure Mouse Anti-Human IgG, Fcγ fragment specific, 1ml
Peroxidase-AffiniPure Mouse Anti-Human IgG, Fc Fragment Specific (min X Bov,Hrs,Ms Sr Prot)